Control:Article Title: IL-4Rα-Expressing B Cells Are Required for CXCL13 Production by Fibroblastic Reticular Cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat anti mouse B220-biotin (Clone RA3-6B2) Life Technologies Cat No# RM2615 Rat anti mouse B220 (Clone RA3-6B2) BioLegend Cat No# 103202 Armenain hamster anti mouse CD3 (Clone 145-2C11) BioLegend Cat No# 100302 Rat anti mouse CD35 (Clone 8C12) BD PharMingen Cat No# 558768 Rat anti mouse ER-TR7 BMA Biomedicals Cat No# T-2109 Rat anti mouse FDCM1 BD PharMingen Cat No# 551320 Armenain hamster anti mouse CD11c (Clone N418) BioLegend Cat No# 117308 Rat anti mouse GL-7-biotin eBiosciences Cat No# 13-5902-85 Rat anti mouse IgD (Clone 11-26c) Southern Biotech Cat No# 1120-01 Rabbit anti mouse laminin Sigma-Aldrich Cat No# L9393 Syrian hamster anti mouse podoplanin (clone 8.1.1) Hybridoma N/A Rat anti mouse CD254 (Clone IKK22/5)-Biotin eBiosciences Cat No# 13-5952-82 Rabbit anti mouse RANKL Peprotech Cat No# 500-P63 Goat anti mouse CXCL13 R&D Systems Cat No# AF470 Goat IgG Control R&D Systems Cat No# AB-108-C Rat anti mouse MAdCAM1 (Clone MECA 367) BioLegend Cat No# 120702 Goat anti armenian hamster-Alexa 594 Jackson Immuno Research Cat No# 127-585-160 Donkey anti Rabbit IgG-Alexa 488 Thermo Fischer Cat No# A21206 Donkey anti Rabbit IgG-Alexa 568 Thermo Fischer Cat No# A10042 Donkey anti Rabbit IgG-Alexa 647 Thermo Fischer Cat No# A31573 Donkey anti Rat IgG-Alexa 488 Thermo Fischer Cat No# A21208 Goat anti Rat IgG-Alexa 568 Thermo Fischer Cat No# A11077 Donkey anti Rat IgG-biotin Jackson Immuno Research Cat No# 712-065-153 Donkey anti Rat IgG-Alexa 647 Jackson Immuno Research Cat No# 712-605-150 Chicken anti Rat IgG-Alexa 647 Thermo Fischer Cat No# A-21472 Goat anti Syrian hamster IgG-Alexa 488 Jackson Immuno Research Cat No# 107-545-142 Goat anti Syrian hamster IgG-Alexa 647 Jackson Immuno Research Cat No# 107-606-142 Rabbit anti Syrian hamster IgG-Alexa 647 Jackson Immuno Research Cat No# 307-605-003 Donkey anti Goat IgG-biotin Jackson Immuno Research Cat No# 705-035-003 Rat anti mouse-CD45 Biolegend Cat No# 103114 Rat anti mouse-CD45 Biolegend Cat No# 103122 Rat anti mouse-CD45 Biolegend Cat No# 103126 Rat anti mouse-CD31 Biolegend Cat No# 102422 Rat anti mouse-CD31 Biolegend Cat No# 102408 Rat anti mouse-CD35 Biolegend Cat No# 123407 Rat anti mouse-CD35 Biolegend Cat No# 123408 Rat anti mouse-CD35 Biolegend Cat No# 123413 Syrian hamster anti mouse Podoplanin eBiosciences Cat No# 50-5381 Rat anti mouse-TER119 Biolegend Cat No# 116215 Rat anti mouse-MAdCAM1 Biolegend Cat No# 120706 Rat anti mouse-MAdCAM1 Biolegend Cat No# 120708 (Continued on next page) Cell Reports 27, 2442–2458.e1–e5, May 21, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological Samples Normal mouse serum Naive mouse (C57BL/6) N/A Normal donkey serum Millipore S30-100 mL Chemicals, Peptides, and Recombinant Proteins Paraformaldehyde Merck-Millipore Cat No# 104005 Collagenase P Roche Cat No# 11215809103 Dispase Roche Cat No# 04942078001 DNase I Roche Cat No# 11284932001 Agarose, low gelling temperature Sigma Aldrich Cat No# A9414 (Ethylenedinitrilo)tetraacetic acid (EDTA) Applichem Cat No# A4892 DAPI Sigma Aldrich Cat No# D9542 Streptavidin-Alexa 488 Molecular Probes Cat No# S-11223 Streptavidin-Alexa 568 Molecular Probes Cat No# S-11226 Streptavidin-Alexa 568 Molecular Probes Cat No# S-21374 ProLongTMGold Antifade Mountant ThermoFisher Cat No# P36934 Tyramide signal amplification kit (Alexa 488 and Alexa 568) ThermoFisher Cat No# T20932/34 Streptavidin HRP Jackson Immunosearch Cat No# 016-030-084 Cobantril McCoy et al., 2008 N/A Tissue-Tek optimum cutting temperature compound (OCT) Fisher Scientific Cat No# 14-373-65 Acetone for HPLC, R 99.9% Sigma Aldrich Cat No# 270725 Critical Commercial Assays Light Cycler 480 SYBR Green I Master mix Roche Cat No# 04707516001 Depleting anti-CD45 microbeads Miltenyi Biotech Cat No# 130-052-301 Depleting anti-TER119 microbeads Miltenyi Biotech Cat No# 130-049-901 Direct-zol RNA MiniPrep kit Zymo Research Cat No# R2051 RevertAid First Strand cDNA Synthesis Kit ThermoFisher Cat No# K1622 Experimental Models: Organisms/Strains Mouse: C57BL/6 (WT) Charles River Strain code: 027 Mouse: JhT knockout Dubey et al., 2016 N/A Mouse: IL-4Ra knockout Dubey et al., 2016 N/A Mouse: LT-b knockout Dubey et al., 2016 N/A Mouse: TCR-bd knockout Dubey et al., 2016 N/A Mouse: C57BL/6N-Tg(Ccl19-Cre)(Ccl19-Cre) Chai et al., 2013 N/A Mouse: Ltbrfl/fl Chai et al., 2013; Dubey et al., 2017 N/A Mouse: Rosa26-eYFP Jackson Laboratories Stock No: 006148 Parasite: Heligmosomoides polygyrus (Hp) Dubey et al., 2016 Dubey et al., 2016 Oligonucleotides Primer for RT PCR Forward (Cxcl13): CATAGATCGGATTCAAGT This paper N/A Primer for RT PCR Reverse (Cxcl13): TCTTGGTCCAGATCACAA This paper N/A Primer for RT PCR Forward (Gapdh): GTGCCAGCCTCGTCCCG This paper N/A Primer for RT PCR Reverse (Gapdh): TTGCCGTGAGTGGAGTCA.
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